|
KMID : 0043320010240040307
|
|
Archives of Pharmacal Research
2001 Volume.24 No. 4 p.307 ~ p.311
|
|
|
Kojic Acid, a Potential Inhibitor of NF-kB Activation in Transfectant Human HaCaT and SCC-73 Cells
|
|
Moon KY
Ahn KS/Lee JS/Kim YS
|
|
|
Abstract
|
|
|
|
The activation of NF-Kb induced by kojic acid, an inhibitor of tyrosinase for biosynthesis of melanin in melanocytes, was investigated in human transfectant HaCaT and SCC-13 cel ls. These two keratinocyte cell lines transfected with psF-Kb-SEAP-NPT plasmic were used to determine the activation of NF-Kb. Transfectant cells release the secretory alkaline phosphatase (SEAP) as a transcription reporter in response to the NF-Kb activity and contain the neomycin phosphotransferase (NPT) gene for the dominant selective marker of genetlcin resistance. NF-Kb activation was measured in the SEAP reporter gene assay using a fluorescence detection method. Kojic acid showed the inhibition of cellular NF-kB activity in both human keratinocyte transfectants. It could also downreRulate the ultraviolet ray (UVR)-induced activation of NF-Kb expression in transfectant HaCaT cells. Moreover the inhibitory activity of kojic acid in transfectant HaCaT cells was found to be more potent than known antioxidants, e.g., vitamin C and N-acetyl-L-cysteine. These results indicate that kojic acid is a potential inhibitor of NF-Kb activation in human keratinocytes, and suggest the hypothesis that NF-kB activation may be involved in kojic acid induced anti-mela- nogenic effect.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|